Review



green multiplex master mix  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    New England Biolabs green multiplex master mix
    Green Multiplex Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/green+multiplex+master+mix/Multiplex+PCR+Master+Mix/pm41981313-338-16-20
    Average 97 stars, based on 362 article reviews
    green multiplex master mix - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Generated:

    Article Title: In vivo base editing rescues liver pathophysiology and peroxisome dysfunction in a mouse model of Zellweger spectrum disorder.
    Article Snippet: Editor sequences were cloned into pT7 expression plasmids (example Addgene number 178113). .. Nature Biomedical Engineering Article https://doi.org/10.1038/s41551-026-01651-5 Linear DNA templates were generated by PCR using the Phusion U Green Multiplex Master Mix (NEB) and purified using the QIAquick PCR Purification kit (Qiagen). .. IVT reactions used a T7 High Yield RNA Synthesis kit (NEB), following the manufacturer’s directions with two exceptions: CleanCap Reagent AG (TriLink BioTechnologies) was added and the uridine-5′-triphosphate in the kit was replaced with N1-methylpseudouridine 5′ triphosphate (TriLink BioTechnologies).

    Polymerase Chain Reaction:

    Article Title: In vivo base editing rescues liver pathophysiology and peroxisome dysfunction in a mouse model of Zellweger spectrum disorder.
    Article Snippet: Editor sequences were cloned into pT7 expression plasmids (example Addgene number 178113). .. Nature Biomedical Engineering Article https://doi.org/10.1038/s41551-026-01651-5 Linear DNA templates were generated by PCR using the Phusion U Green Multiplex Master Mix (NEB) and purified using the QIAquick PCR Purification kit (Qiagen). .. IVT reactions used a T7 High Yield RNA Synthesis kit (NEB), following the manufacturer’s directions with two exceptions: CleanCap Reagent AG (TriLink BioTechnologies) was added and the uridine-5′-triphosphate in the kit was replaced with N1-methylpseudouridine 5′ triphosphate (TriLink BioTechnologies).

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors.
    Article Snippet: For each condition, scaffold integration is the percentage of (number of amplicons with scaffold-templated bases)/(number of reads that align to the amplicon). e10 Cell 186, 3983–4002.e1–e13, August 31, 2023 ll OPEN ACCESSResource In vitro transcription (IVT) of editor mRNA IVT of editor mRNA was performed as described previously.33 Editors were cloned into pT7 expression constructs (example Addgene 178113). .. To generate linear DNA templates for IVT, the pT7-editor plasmids were amplified by PCR using the Phusion U green multiplex master mix (NEB) using primers IVT-fwd and IVT-rev (Table S6B). .. PCRs were purified using the QIAquick PCR purification kit (Qiagen) and eluted in water.

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors
    Article Snippet: Editors were cloned into pT7 expression constructs (example Addgene 178113). .. To generate linear DNA templates for IVT, the pT7-editor plasmids were amplified by PCR using the Phusion U green multiplex master mix (NEB) using primers IVT-fwd and IVT-rev ( B). .. PCRs were purified using the QIAquick PCR purification kit (Qiagen) and eluted in water.

    Multiplex Assay:

    Article Title: In vivo base editing rescues liver pathophysiology and peroxisome dysfunction in a mouse model of Zellweger spectrum disorder.
    Article Snippet: Editor sequences were cloned into pT7 expression plasmids (example Addgene number 178113). .. Nature Biomedical Engineering Article https://doi.org/10.1038/s41551-026-01651-5 Linear DNA templates were generated by PCR using the Phusion U Green Multiplex Master Mix (NEB) and purified using the QIAquick PCR Purification kit (Qiagen). .. IVT reactions used a T7 High Yield RNA Synthesis kit (NEB), following the manufacturer’s directions with two exceptions: CleanCap Reagent AG (TriLink BioTechnologies) was added and the uridine-5′-triphosphate in the kit was replaced with N1-methylpseudouridine 5′ triphosphate (TriLink BioTechnologies).

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors.
    Article Snippet: For each condition, scaffold integration is the percentage of (number of amplicons with scaffold-templated bases)/(number of reads that align to the amplicon). e10 Cell 186, 3983–4002.e1–e13, August 31, 2023 ll OPEN ACCESSResource In vitro transcription (IVT) of editor mRNA IVT of editor mRNA was performed as described previously.33 Editors were cloned into pT7 expression constructs (example Addgene 178113). .. To generate linear DNA templates for IVT, the pT7-editor plasmids were amplified by PCR using the Phusion U green multiplex master mix (NEB) using primers IVT-fwd and IVT-rev (Table S6B). .. PCRs were purified using the QIAquick PCR purification kit (Qiagen) and eluted in water.

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors
    Article Snippet: Editors were cloned into pT7 expression constructs (example Addgene 178113). .. To generate linear DNA templates for IVT, the pT7-editor plasmids were amplified by PCR using the Phusion U green multiplex master mix (NEB) using primers IVT-fwd and IVT-rev ( B). .. PCRs were purified using the QIAquick PCR purification kit (Qiagen) and eluted in water.

    Purification:

    Article Title: In vivo base editing rescues liver pathophysiology and peroxisome dysfunction in a mouse model of Zellweger spectrum disorder.
    Article Snippet: Editor sequences were cloned into pT7 expression plasmids (example Addgene number 178113). .. Nature Biomedical Engineering Article https://doi.org/10.1038/s41551-026-01651-5 Linear DNA templates were generated by PCR using the Phusion U Green Multiplex Master Mix (NEB) and purified using the QIAquick PCR Purification kit (Qiagen). .. IVT reactions used a T7 High Yield RNA Synthesis kit (NEB), following the manufacturer’s directions with two exceptions: CleanCap Reagent AG (TriLink BioTechnologies) was added and the uridine-5′-triphosphate in the kit was replaced with N1-methylpseudouridine 5′ triphosphate (TriLink BioTechnologies).

    Amplification:

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors.
    Article Snippet: For each condition, scaffold integration is the percentage of (number of amplicons with scaffold-templated bases)/(number of reads that align to the amplicon). e10 Cell 186, 3983–4002.e1–e13, August 31, 2023 ll OPEN ACCESSResource In vitro transcription (IVT) of editor mRNA IVT of editor mRNA was performed as described previously.33 Editors were cloned into pT7 expression constructs (example Addgene 178113). .. To generate linear DNA templates for IVT, the pT7-editor plasmids were amplified by PCR using the Phusion U green multiplex master mix (NEB) using primers IVT-fwd and IVT-rev (Table S6B). .. PCRs were purified using the QIAquick PCR purification kit (Qiagen) and eluted in water.

    Article Title: Phage-assisted evolution and protein engineering yield compact, efficient prime editors
    Article Snippet: Editors were cloned into pT7 expression constructs (example Addgene 178113). .. To generate linear DNA templates for IVT, the pT7-editor plasmids were amplified by PCR using the Phusion U green multiplex master mix (NEB) using primers IVT-fwd and IVT-rev ( B). .. PCRs were purified using the QIAquick PCR purification kit (Qiagen) and eluted in water.



    Similar Products

    97
    New England Biolabs green multiplex master mix
    Green Multiplex Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/green+multiplex+master+mix/Multiplex+PCR+Master+Mix/pm41981313-338-16-20
    Average 97 stars, based on 1 article reviews
    green multiplex master mix - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    90
    Thermo Fisher phusion u green multiplex pcr master mix f564l
    Phusion U Green Multiplex Pcr Master Mix F564l, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/green+multiplex+master+mix/phusion+high+fidelity+dna+polymerase/10__1172_slash_jci181879-186-17-24
    Average 90 stars, based on 1 article reviews
    phusion u green multiplex pcr master mix f564l - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    97
    New England Biolabs green master mix
    Green Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/green+multiplex+master+mix/Multiplex+PCR+Master+Mix/pmc12469579-74-40-43
    Average 97 stars, based on 1 article reviews
    green master mix - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    90
    NZYTech Inc pcr master mix multiplex pcr nzytaq 2x green master mix
    Pcr Master Mix Multiplex Pcr Nzytaq 2x Green Master Mix, supplied by NZYTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/green+multiplex+master+mix/multiplex+pcr+nzytaq+2x+colourless+master+mix/10__3390_slash_microbiolres16060133-95-19-29
    Average 90 stars, based on 1 article reviews
    pcr master mix multiplex pcr nzytaq 2x green master mix - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher phusion u green multiplex pcr master mix
    Phusion U Green Multiplex Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/green+multiplex+master+mix/phusion+high+fidelity+dna+polymerase/pmc12165785-181-15-22
    Average 90 stars, based on 1 article reviews
    phusion u green multiplex pcr master mix - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results